Purpose
This
SOP describes the procedure for procedure for HPLC column washing.
Scope
This
SOP is applicable for HPLC column washing at ABC Limited.
HPLC column washing procedure for mobile phase
containing buffer:
- After elution of last injection, run the Mobile phase for 10 minutes.
- Flush the injector with sufficient amount of Milli-Q water.
- Run the Milli-Q water (Degassed) through HPLC column by 0.5 ml/min – 1.5ml/min flow where by pressure will not exceed above 3500 psi.
- Flush the column with Milli-Q water for 30 minutes at 1.0 ml/min.
- Prepare a solvent mixture of Acetonitrile or methanol (70 %) and (30 %) Milli-Q water and flush column for 30 minutes 1.0 ml/min..
- Store the column with end fitting with respective column box.
- In case there is any problem in Data, inform to IT and QA for corrective action.
HPLC
Column washing procedure for Mobile Phase (normal phase):
- After elution of last injection, run the Mobile Phase for 10 minutes
- Flush the injector with sufficient amount of HPLC grade IPA.
- Run the HPLC grade IPA through HPLC column by 0.5ml/min to 1.5-ml/min flow where by pressure will not exceed above 3500 psi.
- Flush the column with HPLC grade IPA for 45 minutes 1.0 ml/min.
- Store the column with end fitting In Column safe
