Purpose
This
SOP describes the procedure for Preparation and Standardization of Volumetric Solutions.
Procedure:
- Use Milli Q Water for preparation of volumetric solution.
- Wear surgical gloves during preparation of volumetric solutions.
- Take appropriate glassware of suitable capacity to prepare the volumetric solutions.
- Use AR & LR Grade chemicals for the preparation of volumetric Solutions.
- Ensure that chemical is within ‘use before date’ from the sticker on the container.
- Store all the solutions in cleaned dry glass bottles. Store light sensitive solutions in amber color glass bottle.
- Prepare volumetric solutions as per IP/ /BP/USP /EP or respective In-house standard test procedure for that specified volumetric solution.
- Prepare the volumetric solutions after every one month or if physical appearance changes. Standardize the solution immediately after preparation before use and re-standardized once in 15 days. Frequency of preparation and standardization of volumetric solutions is given in their respective standard test procedure.
- Fill the label of the volumetric solution
with following details and affix it on the container.
- Documentation maintain in “Raw Data Record of Volumetric Solution and “Record of volumetric solution Preparation”.
- Paste a label on the side of reagent bottle with Name, Molarity/Normality, Date of Preparation, Date of Standardization, Date of Re-standardization, and Use before date, Prepared By and Checked By.
- Standardize the volumetric solution in triplicate.
- The RSD of three analysis should be not more than 0.2%.
- Use average of 3 values for calculation.
- Before signing the records, In-charge checks all calculations for correctness and Ensures that solutions are prepared as per standard procedure.
